I left the chromatography paper in the solvent until all the solvent had been absorbed, approximately 2 to 3 minutes I removed them from the solvent, marked the solvent line and set them aside to dry. For the experiment with the rubbing alcohol I also placed a drop of each dye, but used a paper coffee filter.
If, at this point, the color of your liquid is too faint, you might want to remove the lid of the saucepan and continue boiling until some liquid has evaporated and a more concentrated color is obtained. Hang your strips to dry in the empty glasses or on a drying rack.
Your chromatography setup should look similar to this example. Remove the candy, then dip a pipet tip, or clean wooden splint tip, into the now-colored drop of water.
Make sure the two strips do not touch each other or the beaker and that their bottoms are aligned. Repeat steps 6b to 6g with four more strips and four new candies that are the same type and color e.
Hang the strip in one of the glasses that is partially filled with water by letting the pencil rest on the glass rim. Turmeric is a spice, a dye, and a medicinal herb. Use a pencil to mark how far the solvent rose. Keep a close eye on your chromatography strip and the solvent front— if you let it run too long the dye may run off the paper and become distorted.
Marker companies combine a small subset of color molecules to make a wide range of colors, much like you can mix paints to make different colors. If necessary, add more of the salt solution. Most watercolor marker inks are colored with synthetic color molecules.
Leave the candy in the drop of water for three minutes to allow the dye to dissolve. Repeat step 6 for the other two colors of candy you want to test.
Repeat steps 8c to 8d until you have 15 chromatography strips spotted with food dye— 5 red, 5 blue, and 5 green. Leave the first strip in its glass as you repeat the previous two steps with the second strip and the second glass.
Analyzing Your Results Create a data table like Table 1 for each candy type and color or food coloring that you tested in your lab notebook. Take the brown marker and a paper strip and draw a short line about one centimeter on the middle section of the pencil line.
Dip a clean wooden splint tip or a pipet into the drop of food coloring. Cut each filter paper in half length-wise to make approximately 2 centimeters cm wide by 7. Squeeze the pipet at its widest point. Repeat steps 6e to 6f three more times. Leave the candy in the drop of water for three minutes to allow the dye to dissolve.Paper Chromatography of Food Dyes and Colors Revision F8 Page 3 of 9 Procedure Part A.
Equipment and Set Up A1. Obtain 3 pieces of 11 by 18 cm Whatman # 1 Chromatography paper. A2. Using a pencil, draw an origin line that is 2 cm from the paper edge along the long side.
See diagram at right. A3. To investigate the color components of this dye, repeat the previous procedure but replace the marker line with a drop of natural dye. A dropper will help create a nice drop. Let the drop of dye. Food Dye Chromatography continued 3 21 F et ght eered When the solvent is within 1–2 cm of the top of the paper, remove it from the beaker.
With a pencil, lightly draw a line to mark the distance the solvent traveled to the top of the chromatography paper. This is called the solvent front. Gently remove the staples and lay the paper flat. In this food science project, you will use the R f value to compare the "unknown" components of colored candy dyes with the "known" components of food coloring dyes.
Since there are only a small number of approved food dyes, you should be able to identify the ones used in the candies by comparison to the chromatography results for food.
Chromatography of Food Dye Chem DL3 Abstract: The goal of this experiment was to use different dyes to determine the Rf value. Chromatography is done to determine the presence of a mixture in a substance.
Food dye molecules that are more highly charged, or have more ionic binding sites, are more polar and will be attracted to the paper more. So they will have lower Rf values. The distance a sample moves along the chromatography paper is compared to the overall distance the solvent travels which is the ratio is called of Rf or rate of flow.Download